Exceptionally high heat flux needed to sustain the Northeast Greenland Ice Stream

The Northeast Greenland Ice Stream (NEGIS) currently drains more than 10 % of the Greenland Ice Sheet area and has recently undergone significant dynamic changes. It is therefore critical to accurately represent this feature when assessing the future contribution of Greenland to sea level rise. At present, NEGIS is reproduced in ice sheet models by inferring basal conditions using observed surface velocities. This approach helps estimate conditions at the base of the ice sheet but cannot be used to estimate the evolution of basal drag in time, so it is not a good representation of the evolution of the ice sheet in future climate warming scenarios. NEGIS is suggested to be initiated by a geothermal heat flux anomaly close to the ice divide, left behind by the movement of Greenland over the Icelandic plume. However, the heat flux underneath the ice sheet is largely unknown, except for a few direct measurements from deep ice core drill sites. Using the Ice Sheet System Model (ISSM), with ice dynamics coupled to a subglacial hydrology model, we investigate the possibility of initiating NEGIS by inserting heat flux anomalies with various locations and intensities. In our model experiment, a minimum heat flux value of 970 mW m−2 located close to the East Greenland Ice-core Project (EGRIP) is required locally to reproduce the observed NEGIS velocities, giving basal melt rates consistent with previous estimates. The value cannot be attributed to geothermal heat flux alone and we suggest hydrothermal circulation as a potential explanation for the high local heat flux. By including high heat flux and the effect of water on sliding, we successfully reproduce the main characteristics of NEGIS in an ice sheet model without using data assimilation.publishedVersio

Sensitivity Study of Four Land Surface Schemes in the WRF Model

The Weather Research and Forecasting (WRF) model version 3.0 developed by the National Center for Atmospheric Research (NCAR) includes three land surface schemes: the simple soil thermal diffusion (STD) scheme, the Noah scheme, and the Rapid Update Cycle (RUC) scheme. We have recently coupled the sophisticated NCAR Community Land Model version 3 (CLM3) into WRF to better characterize land surface processes. Among these four land surface schemes, the STD scheme is the simplest in both structure and process physics. The Noah and RUC schemes are at the intermediate level of complexity. CLM3 includes the most sophisticated snow, soil, and vegetation physics among these land surface schemes. WRF simulations with all four land surface schemes over the western United States (WUS) were carried out for the 1 October 1995 through 30 September 1996. The results show that land surface processes strongly affect temperature simulations over the (WUS). As compared to observations, WRF-CLM3 with the highest complexity level significantly improves temperature simulations, except for the wintertime maximum temperature. Precipitation is dramatically overestimated by WRF with all four land surface schemes over the (WUS) analyzed in this study and does not show a close relationship with land surface processes

Infection patterns and fitness effects of Rickettsia and Sodalis symbionts in the green lacewing Chrysoperla carnea

Endosymbionts are widely distributed in insects and can strongly affect their host ecology. The common green lacewing (Chrysoperla carnea) is a neuropteran insect which is widely used in biological pest control. However, their endosymbionts and their interactions with their hosts have not been very well studied. Therefore, we screened for endosymbionts in natural and laboratory populations of Ch. carnea using diagnostic PCR amplicons. We found the endosymbiont Rickettsia to be very common in all screened natural and laboratory populations, while a hitherto uncharacterized Sodalis strain was found only in laboratory populations. By establishing lacewing lines with no, single or co-infections of Sodalis and Rickettsia, we found a high vertical transmission rate for both endosymbionts (>89%). However, we were only able to estimate these numbers for co-infected lacewings. Sodalis negatively affected the reproductive success in single and co-infected Ch. carnea, while Rickettsia showed no effect. We hypothesize that the fitness costs accrued by Sodalis infections might be more tolerable in the laboratory than in natural populations, as the latter are also prone to fluctuating environmental conditions and natural enemies. The economic and ecological importance of lacewings in biological pest control warrants a more profound understanding of its biology, which might be influenced by symbionts

Cochlear implantation is safe and effective in patients with MYH9-related disease

Background: MYH9-related disease (MYH9-RD) is a rare syndromic disorder deriving from mutations in MYH9, the gene for the heavy chain of non-muscle myosin IIA. Patients present with congenital thrombocytopenia and giant platelets and have a variable risk of developing sensorineural deafness, kidney damage, presenile cataract, and liver abnormalities. Almost all MYH9-RD patients develop the hearing defect, which, in many individuals, progresses to severe to profound deafness with high impact on quality of life. These patients are potential candidates for cochlear implantation (CI), however, no consistent data are available about the risk to benefit ratio of CI in MYH9-RD. The only reported patient who received CI experienced perisurgery complications that have been attributed to concurrent platelet defects and/or MYH9 protein dysfunction. Methods: By international co-operative study, we report the clinical outcome of 10 patients with MYH9-RD and severe to profound deafness who received a CI at 8 institutions. Results: Nine patients benefited from CI: in particular, eight of them obtained excellent performances with restoration of a practically normal hearing function and verbal communication abilities. One patient had a slightly worse performance that could be explained by the very long duration of severe deafness before CI. Finally, one patient did not significantly benefit from CI. No adverse events attributable to MYH9-RD syndrome were observed, in particular no perisurgery bleeding complications due to the platelet defects were seen. Patients' perioperative management is described and discussed. Conclusions: CI is safe and effective in most patients with MYH9-RD and severe to profound deafness and should be offered to these subjects, possibly as soon as they develop the criteria for candidacy

Intrabiliary infusion of naked DNA vectors targets periportal hepatocytes in mice

Hydrodynamic tail vein injection (HTV) is the "gold standard" for delivering naked DNA vectors to mouse liver, thereby transfecting predominately perivenous hepatocytes. While HTV corrects metabolic liver defects such as phenylketonuria or cystathionine β-synthase deficiency, correction of spf ash mice with ornithine transcarbamylase (OTC) deficiency was not possible despite overexpression in the liver, as the OTC enzyme is primarily expressed in periportal hepatocytes. To target periportal hepatocytes, we established hydrodynamic retrograde intrabiliary injection (HRII) in mice and optimized minicircle (MC) vector delivery using luciferase as a marker gene. HRII resulted in a transfection efficiency below 1%, 100-fold lower than HTV. While HRII induced minimal liver toxicity compared with HTV, overexpression of luciferase by both methods, but not of a natural liver-specific enzyme, elicited an immune response that led to the elimination of luciferase expression. Further testing of MC vectors delivered via HRII in spf ash mice did not result in sufficient therapeutic efficacy and needs further optimization and/or selection of the corrected cells. This study reveals that luciferase expression is toxic for the liver. Furthermore, physical delivery of MC vectors via the bile duct has the potential to treat defects restricted to periportal hepatocytes, which opens new doors for non-viral liver-directed gene therapy. Keywords: FLAG-tagged OTC; OTC deficiency; hydrodynamic retrograde intrabiliary injection; hydrodynamic tail vein injection; immune response; liver toxicity; liver-directed gene therapy; luciferase expression; naked DNA minicircle vector; non-viral; urea cycle disorder

An Initial Survey of White Dwarfs in the Sloan Digital Sky Survey

An initial assessment is made of white dwarf and hot subdwarf stars observed in the Sloan Digital Sky Survey. In a small area of sky (190 square degrees), observed much like the full survey will be, 269 white dwarfs and 56 hot subdwarfs are identified spectroscopically where only 44 white dwarfs and 5 hot subdwarfs were known previously. Most are ordinary DA (hydrogen atmosphere) and DB (helium) types. In addition, in the full survey to date, a number of WDs have been found with uncommon spectral types. Among these are blue DQ stars displaying lines of atomic carbon; red DQ stars showing molecular bands of C_2 with a wide variety of strengths; DZ stars where Ca and occasionally Mg, Na, and/or Fe lines are detected; and magnetic WDs with a wide range of magnetic field strengths in DA, DB, DQ, and (probably) DZ spectral types. Photometry alone allows identification of stars hotter than 12000 K, and the density of these stars for 15<g<20 is found to be ~2.2 deg^{-2} at Galactic latitudes 29-62 deg. Spectra are obtained for roughly half of these hot stars. The spectra show that, for 15<g<17, 40% of hot stars are WDs and the fraction of WDs rises to ~90% at g=20. The remainder are hot sdB and sdO stars.Comment: Accepted for AJ; 43 pages, including 12 figures and 5 table

Evolution of the Color-Magnitude Relation in Galaxy Clusters at z ~1 from the ACS Intermediate Redshift Cluster Survey

We apply detailed observations of the Color-Magnitude Relation (CMR) with the ACS/HST to study galaxy evolution in eight clusters at z~1. The early-type red sequence is well defined and elliptical and lenticular galaxies lie on similar CMRs. We analyze CMR parameters as a function of redshift, galaxy properties and cluster mass. For bright galaxies (M_B < -21mag), the CMR scatter of the elliptical population in cluster cores is smaller than that of the S0 population, although the two become similar at faint magnitudes. While the bright S0 population consistently shows larger scatter than the ellipticals, the scatter of the latter increases in the peripheral cluster regions. If we interpret these results as due to age differences, bright elliptical galaxies in cluster cores are on average older than S0 galaxies and peripheral elliptical galaxies (by about 0.5Gyr). CMR zero point, slope, and scatter in the (U-B)_z=0 rest-frame show no significant evolution out to redshift z~1.3 nor significant dependence on cluster mass. Two of our clusters display CMR zero points that are redder (by ~2sigma) than the average (U-B)_z=0 of our sample. We also analyze the fraction of morphological early-type and late-type galaxies on the red sequence. We find that, while in the majority of the clusters most (80% to 90%) of the CMR population is composed of early-type galaxies, in the highest redshift, low mass cluster of our sample, the CMR late-type/early-type fractions are similar (~50%), with most of the late-type population composed of galaxies classified as S0/a. This trend is not correlated with the cluster's X-ray luminosity, nor with its velocity dispersion, and could be a real evolution with redshift.Comment: ApJ, in press, 27 pages, 22 figure